LC-MS Introduction

LC-MS is a hyphenated technique, which combines the separating power of high performance liquid chromatography (HPLC), with the detection power of mass spectrometry (MS). LC-MS covers a broad range of application areas. This module explores the instrument acquisition methods used and examines the type of data that can be produced from such systems.

Topics include:

• Definitions
• Instrument fundamentals
• LC-MS process
• Why and when to use LC-MS
• Ionization overview
• Atmospheric pressure ionization (API)
• Electrospray ionization (ESI)
• Atomospheric pressure chemical ionization (APCI)
• Atomospheric pressure photoionization (APPI)
• Mass analyzers
• Quadrupole mass analyzer
• Time-of-flight mass analyzer
• Ion trap mass analyzer
• Tandem mass spectrometry (MS/MS)
• Detectors