LC‑MS: where separation meets identification – unlock the power of the hyphen.
LC‑MS USERS
BASIC LC KNOWLEDGE
INTERMEDIATE
PHARMA · ENVIRO · FOOD
- Designed for: users of LC‑MS and LC‑MS‑MS equipment, and anyone interested in the analytical capabilities of these instruments
- Ideal for analysts, technicians, and method developers
- Prerequisite: no prior MS knowledge needed, but a basic understanding of liquid chromatography is beneficial
- Familiarity with general laboratory practices recommended
Learning outcomes
- Understand how modern API designs work and which one best fits your LC and application requirements
- Learn how ions are produced and how to optimise LC mobile phase and interface parameters for highest sensitivity, accuracy and reproducibility
- Explore the anatomy and working principles of quadrupoles, ion traps, time‑of‑flight detectors and more
- Master the information that can be acquired in MS, MS‑MS and MSⁿ modes, and optimise instrument settings for high data quality
Introduction to LC‑MS – Fundamentals & Instrumentation
LIVE ONLINE INTERACTIVE SESSION · one‑day essentials
We have distilled critical knowledge of liquid chromatography and mass spectrometry into a one‑day, interactive course. Learn the absolute essentials – from atmospheric pressure interface (API) designs to ion suppression, electrospray, APCI, and MS‑MS data acquisition – and implement new ideas immediately with our unique multi‑media examples and tutorial exercises.
🔬 BASICS OF LC‑MS
- What is LC‑MS? – definitions & principles
- How LC and MS work together
- Key applications: pharmaceuticals, environmental science, food safety
⚙️ LC‑MS INSTRUMENTATION
Liquid Chromatography (LC) overview
- Role of LC in LC‑MS
- Key components: pump, injector, column, detector
- Reversed‑phase vs. normal‑phase chromatography
Mass Spectrometry (MS) fundamentals
- Ionization process & principles
- The role of vacuum and ion optics
- Mass‑to‑charge ratio (m/z) basics
⚡ IONIZATION TECHNIQUES IN LC‑MS
Atmospheric Pressure Ionization (API) methods
- Electrospray Ionization (ESI): best for polar compounds, peptides, proteins
- Atmospheric Pressure Chemical Ionization (APCI): suitable for non‑polar compounds
- Atmospheric Pressure Photoionization (APPI): for very non‑polar analytes
🧪 MASS ANALYZERS & DETECTION
Types of mass analyzers
- Quadrupole: used in single & triple quadrupole MS
- Time‑of‑Flight (TOF): high resolution, accurate mass
- Ion Trap & Orbitrap: high sensitivity and multi‑stage MS capabilities
- Tandem Mass Spectrometry (MS/MS): fragmentation techniques for structural analysis
Detectors in LC‑MS
- Electron multiplier detectors
- Time‑of‑flight (TOF) detection principles
📊 APPLICATION AREAS OF LC‑MS
- Drug discovery & metabolite identification
- Environmental analysis & food safety
- Proteomics & biomarker research
🗣️ Q&A AND CLOSING DISCUSSION
- Summary of key concepts
- Open discussion on challenges faced in LC‑MS
- Resources for further learning
💻 ONLINE PRACTICAL COMPONENT
- Interactive API optimisation exercises
- Virtual mass analyzer comparison
- Case studies: method development for pharmaceuticals / environmental contaminants
ESI · APCI · APPI
Quadrupole · TOF · Ion trap
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